An improved method for the preparation of protective pseudoglobulin.

Experiments indicating the existence of a menstrual toxin responsible for some of the clinical features of preeclamptic and eclamptic toxemias of pregnancy, and of a protective factor capable of neutralizing this toxin in animal experiments and in human cases have been reported by Smith and Smith (l-3). The apparent success obtained in some of the clinical cases in which protective pseudoglobulin (PPs) was administered indicated the desirability of a method for the preparation of the material in higher yield and purity. The work of Cohn and his colleagues on the fractionation of human plasma in ethanol-water mixtures at low temperature, advantage being taken of pH and ionic strength, offered a means whereby this end might be accomplished.